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Rad9 Mouse mAb
Datasheet   

  • Catalog Number:

    E2200784

  • Amount:

    100ul

  • Source:

    Mouse

  • Form of Antibody:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Specificity/Sensitivity:

    This antibody detects endogenous levels of RAD9A,and does not cross-react with related proteins.

  • Entrez summary:

    This gene product is highly similar to Schizosaccharomyces pombe rad9,a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair.This protein possesses 3´ to 5´ exonuclease activity,which may contribute to its role in sensing and repairing DNA damage.It forms a checkpoint protein complex with RAD1 and HUS1.This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage,which is thought to be important for triggering the checkpoint-signaling cascade.Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

  • UniPort summary Function:

    Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair.The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C(RFC)clamp loader complex.Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair(LP-BER).The 9-1-1 complex stimulates DNA polymerase beta(POLB)activity by increasing its affinity for the 3´-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds;endonuclease FEN1 cleavage activity on substrates with double,nick,or gap flaps of distinct sequences and lengths;and DNA ligase I(LIG1)on long-patch base excision repair substrates.The 9-1-1 complex is necessary for the recruitment of C12orf32/RHINO to sites of double-stranded breaks(DSB)occurring during the S phase.RAD9A possesses 3´->5´ double stranded DNA exonuclease activity.Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

  • Immunogen:

    Purified recombinant human RAD9A protein fragments expressed in E.coli

  • Antibody type:

    Monoclonal Antibody

  • Isotype:

    IgG2b

  • Purification:

    Affinity Purified

  • Cellular localization:

    Nucleus

  • Reactivity:

    Human,Mouse,Rat,Monkey

  • Applications:

    WB,IP

  • Molecular Weight:

    Calculated MW: 43 kDa; Observed MW: 60 kDa

  • UniProt Number:

    Q99638

  • Gene ID:

    5883

  • Gene symbol:

    RAD9

  • Altername:

    RAD9; hRAD9

  • Western blot analysis of RAD9A in Hela, MCF-7, 3T3, COS7 and rat testis lysates using RAD9A antibody.

  • Immunoprecipitation analysis of Rad9 in Hela and 3T3 lysates using RAD9A antibody.

  • Price:

    255$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

  • Product Type:

    Primary Antibody

  • Background:

    This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

  • Clone Number:

    3A3-A7-F8

  • Gene Name:

    RAD9A

  • Recommended Dilutions:

    WB: 1/500-1/1000 IP: 1/20

  • Swiss-Prot No.:

    Q99638

  • Storage/Stability:

    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

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Catalog Number

  • Amount:100ul
  • Price:255$

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Rad9 Mouse mAb | EnoGene Biotech Co. Ltd