World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
Rb Polyclonal Antibody
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
BackgroundThe retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
Product Information
SpeciesRabbit
IsotypeIgG
Molecular Weight110kDa
Storage/StabilityStore at -20oC or -80oC. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
All Names
Alternameretinoblastoma 1; RB1; OSRC; RB; p105-Rb; pRb; pp110
ImmunogenFusion protein of human Rb
PurificationAffinity purification
Gene ID5925
References1.Sherr, C.J. (1996) Science 274, 1672-7. 2.Nevins, J.R. (1992) Science 258, 424-9. 3.Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90. 4.Hu, Q.J. et al. (1990) EMBO J 9, 1147-55. 5.Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83. 6.Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61. 7.Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301. 8.Kitagawa, M. et al. (1996) EMBO J 15, 7060-9. 9.Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.
WB 1:500 - 1:1000
IHC 1:50 - 1:100
WB 1:500 - 1:1000
IHC 1:50 - 1:100
tyrosine Western blot analysis of Jurkat cell cycle-dependent and A431 cell cycle requires cell using Rb antibody. E-CDK2 observed
Conjugated Antibody
This product is also available conjugated. Format: E90003-Conjugate. Price: $485 / Size: 100μl.
E90003-FITC$485 / 100μlE90003-Biotin$485 / 100μlE90003-HRP$485 / 100μlE90003-AP$485 / 100μlE90003-AF350$485 / 100μlE90003-AF405M$485 / 100μlE90003-AF488$485 / 100μlE90003-AF514$485 / 100μlE90003-AF532$485 / 100μlE90003-AF555$485 / 100μlE90003-AF568$485 / 100μlE90003-AF405S$485 / 100μlE90003-AF405L$485 / 100μlE90003-AF546$485 / 100μlE90003-AF594$485 / 100μlE90003-AF610$485 / 100μlE90003-AF635$485 / 100μlE90003-AF647$485 / 100μlE90003-AF680$485 / 100μlE90003-AF700$485 / 100μlE90003-AF750$485 / 100μlE90003-AF790$485 / 100μlE90003-APC$485 / 100μlE90003-PE$485 / 100μlE90003-Cy3$485 / 100μlE90003-Cy5$485 / 100μlE90003-Cy5.5$485 / 100μlE90003-Cy7$485 / 100μlE90003-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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