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Primary Antibodies

Raf1 (Phospho-Ser259) Antibody

E011006-1Cite ID: EN0000010DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
P04049
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Raf-1 around the phosphorylation site of serine 259 (S-T-SP-T-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityRaf-1 (phospho-Ser259) antibody detects endogenous levels of Raf-1 only when phosphorylated at serine 259.
ReferencesDougherty M K, et al. (2005) Mol Cell. 17(2): 215-224.
Hekman M, et al. (2005) FEBS Lett. 579(2): 464-468.
Avruch J,et al. (1994) Trends Biochem. Sci. 19, 279-283.
Chong H, et al. (2001) EMBO J. 20, 3716-3727.
King A J, et al. (1998) Nature. 396:180-183.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf-1 (phospho-Ser259) antibody (E011006).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf-1 (phospho-Ser259) antibody (E011006).
Western blot analysis using Raf-1 (Ab-259) antibody (E021006, Line 1, 2, and 3) and Raf-1 (phospho- Ser259) antibody (E011006, Line 4, 5, and 6).
Western blot analysis using Raf-1 (Ab-259) antibody (E021006, Line 1, 2, and 3) and Raf-1 (phospho- Ser259) antibody (E011006, Line 4, 5, and 6).

Conjugated Antibody

This product is also available conjugated. Format: E011006-1-Conjugate. Price: $485 / Size: 100μl.

E011006-1-FITC$485 / 100μlE011006-1-Biotin$485 / 100μlE011006-1-HRP$485 / 100μlE011006-1-AP$485 / 100μlE011006-1-AF350$485 / 100μlE011006-1-AF405M$485 / 100μlE011006-1-AF488$485 / 100μlE011006-1-AF514$485 / 100μlE011006-1-AF532$485 / 100μlE011006-1-AF555$485 / 100μlE011006-1-AF568$485 / 100μlE011006-1-AF405S$485 / 100μlE011006-1-AF405L$485 / 100μlE011006-1-AF546$485 / 100μlE011006-1-AF594$485 / 100μlE011006-1-AF610$485 / 100μlE011006-1-AF635$485 / 100μlE011006-1-AF647$485 / 100μlE011006-1-AF680$485 / 100μlE011006-1-AF700$485 / 100μlE011006-1-AF750$485 / 100μlE011006-1-AF790$485 / 100μlE011006-1-APC$485 / 100μlE011006-1-PE$485 / 100μlE011006-1-Cy3$485 / 100μlE011006-1-Cy5$485 / 100μlE011006-1-Cy5.5$485 / 100μlE011006-1-Cy7$485 / 100μlE011006-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

Raf1 (Phospho-Ser259) Antibody | EnoGene Biotech Co. Ltd