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Pyruvate Dehydrogenase E2 (4A4) Mouse mAb
Datasheet   

  • Catalog Number:

    E2200678

  • Amount:

    100ul

  • Source:

    Mouse

  • Form of Antibody:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Specificity/Sensitivity:

    This antibody detects endogenous levels of DLAT and does not cross-react with related proteins.

  • Entrez summary:

    This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood

  • UniPort summary Function:

    he pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

  • Immunogen:

    Purified recombinant human Pyruvate Dehydrogenase E2 protein fragments expressed in E.coli.

  • Antibody type:

    Monoclonal Antibody

  • Isotype:

    IgG1

  • Purification:

    Affinity Purified

  • Cellular localization:

    Mitochondrion matrix.

  • Reactivity:

    Human,Mouse

  • Applications:

    WB,ICC/IF,IP

  • Molecular Weight:

    Calculated MW: 69 kDa; Observed MW: 69 kDa

  • UniProt Number:

    P10515

  • Gene ID:

    1737

  • Gene symbol:

    DLTA; PDCE2; PDC-E2

  • Altername:

    DLAT; DLTA; E2; PBC; PDCE2

  • Western blot analysis of Pyruvate Dehydrogenase E2 in Jurkat, A549, U251, F9, Lncap and Hela lysates using Pyruvate Dehydrogenase E2 antibody.

  • Price:

    255$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

  • Product Type:

    Primary Antibody

  • Background:

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

  • Clone Number:

    4A4-B6-C10

  • Gene Name:

    DLAT

  • Recommended Dilutions:

    WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20

  • Swiss-Prot No.:

    P10515

  • Storage/Stability:

    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

  • Western blot detection of DLAT in Jurkat, A549, U251, F9,LNCAP&Hela cell lysates using DLAT antibody (1:1000 diluted). Predicted band size:69KDa. Observed band size:69KDa.

  • Immunoprecipitation analysis of Hela cell lysates using DLAT antibody.

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Catalog Number

  • Amount:100ul
  • Price:255$

Add to Cart

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Pyruvate Dehydrogenase E2 (4A4) Mouse mAb | EnoGene Biotech Co. Ltd