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PTK2B Antibody
Datasheet   

  • Catalog Number:

    E2200625

  • Amount:

    100ul

  • Source:

    Mouse

  • Form of Antibody:

    Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol

  • Specificity/Sensitivity:

    This antibody detects endogenous levels of PTK2B and does not cross-react with related proteins.

  • Entrez summary:

    This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.

  • UniPort summary Function:

    Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at ´Tyr-9, ´Tyr-373´, and ´Tyr-376´. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca2+ levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.

  • Immunogen:

    Purified recombinant human PTK2B protein fragments expressed in E.coli.

  • Antibody type:

    Monoclonal antibody

  • Isotype:

    IgG2a

  • Purification:

    Affinity purified

  • Cellular localization:

    Cytoplasm

  • Reactivity:

    H,

  • Applications:

    WB

  • Molecular Weight:

    116kDa

  • UniProt Number:

    Q14289

  • Gene ID:

    NM_173176.1

  • Gene symbol:

    PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK

  • Altername:

    Calcium-dependent tyrosine kinase Short name=CADTK Calcium-regulated non-receptor proline-rich tyrosine kinase Cell adhesion kinase beta Short name=CAK-beta Short name=CAKB Focal adhesion kinase 2 Short name=FADK 2 Proline-rich tyrosine kinase 2 Related adhesion focal tyrosine kinase Short name=RAFTK

  • Immunocytochemistry stain of Hela using AKR1B1 antibody (1:300).

  • Western blot detection of AKR1B1 in HT1080, MCF7, A431&Hela cell lysates using AKR1B1 antibody (1:1000 diluted). Predicted band size:36KDa. Observed band size:36KDa.

  • Price:

    255$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

  • Storage/Stability:

    Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

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Catalog Number

  • Amount:100ul
  • Price:255$

Add to Cart

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PTK2B Antibody | EnoGene Biotech Co. Ltd