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Aurora A (2E3) Mouse mAb
Datasheet   

  • Catalog Number:

    E2200525

  • Amount:

    100ul

  • Source:

    Mouse

  • Form of Antibody:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Specificity/Sensitivity:

    This antibody detects endogenous levels of AURKA(N-term) and does not cross-react with related proteins.

  • Entrez summary:

    The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

  • UniPort summary Function:

    Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizating p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.

  • Immunogen:

    Purified recombinant human Aurora Kinase A protein fragments expressed in E.coli.

  • Antibody type:

    Monoclonal Antibody

  • Isotype:

    IgG1

  • Purification:

    Affinity Purified

  • Cellular localization:

    Cytoplasm.

  • Reactivity:

    Human,Monkey

  • Applications:

    WB,ICC/IF

  • Molecular Weight:

    Calculated MW: 46 kDa; Observed MW: 46 kDa

  • UniProt Number:

    O14965

  • Gene ID:

    6790

  • Gene symbol:

    AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; PPP1R47

  • Altername:

    AIK; ARK1; AYK1; Aurora-A; Aurora-related kinase 1; BTAK; IAK1; Ipl1- and aurora-related kinase 1; STK15; STK6; Serine/threonine kinase 15

  • Western blot analysis of Aurora Kinase A in SW480 and COS7 lysates using Aurora Kinase A
    antibody.

  • Price:

    255$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

  • Product Type:

    Primary Antibody

  • Background:

    Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis.

  • Clone Number:

    2E3-C10-C11-G6

  • Gene Name:

    AURKA

  • Recommended Dilutions:

    WB: 1/500-1/1000 IF: 1/50-1/200

  • Swiss-Prot No.:

    O14965

  • Storage/Stability:

    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

  • Immunofluorescence analysis of Aurora A (2E3) in Hela cells using Aurora Kinase A
    antibody(green) and Histone H3.1 (Phospho-Ser1) antibody (red),and DAPI (blue)

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Catalog Number

  • Amount:100ul
  • Price:255$

Add to Cart

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Aurora A (2E3) Mouse mAb | EnoGene Biotech Co. Ltd