You are here: EnoGene Home > Products > Pathological (Immunohistochemistry, IHC) antibodies > BRG1 (4E11) Mouse mAb

BRG1 (4E11) Mouse mAb
Datasheet   

  • Catalog Number:

    E2200282

  • Amount:

    100ul

  • Source:

    Mouse

  • Form of Antibody:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Specificity/Sensitivity:

    This antibody detects endogenous levels of SMARCA4 and does not cross-react with related proteins.

  • Entrez summary:

    The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Multiple transcript variants encoding different isoforms have been found for this gene

  • UniPort summary Function:

    Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues By similarity. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.

  • Immunogen:

    Purified recombinant human BRG1 protein fragments expressed in E.coli.

  • Antibody type:

    Monoclonal Antibody

  • Isotype:

    IgG1

  • Purification:

    Affinity Purified

  • Cellular localization:

    Nucleus

  • Reactivity:

    Human

  • Applications:

    WB,IP

  • Molecular Weight:

    Calculated MW: 185 kDa; Observed MW: 220 kDa

  • UniProt Number:

    P51532

  • Gene ID:

    6597

  • Gene symbol:

    BRG1; SNF2; SWI2; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; SNF2-BETA

  • Altername:

    SMARCA4; BAF190A; BRG1; SNF2B; SNF2L4; Transcription activator BRG1; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; SNF2-beta; SWI/S

  • Western blot analysis of SBRG1 in Hela NE and K562 lysates using BRG1 antibody.

  • Price:

    255$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

  • Product Type:

    Primary Antibody

  • Background:

    ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.

  • Clone Number:

    4E11-E11-G11

  • Gene Name:

    SMARCA4

  • Recommended Dilutions:

    WB: 1/500-1/1000 IP: 1/20

  • Swiss-Prot No.:

    P51532

  • Storage/Stability:

    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Add to Cart

Related Products

c-Jun (Phospho-Ser63) Antibody
c-Jun (Phospho-Ser63) Antibody Datasheet  COA   

  • Catalog Number:

    E011001-1

  • Amount:

    50μg/50μl

  • Reactivity:

    Human, Mouse

  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100

  • Swiss-Prot No.:

    P05412

  • Price:

    145$
  • Add to Cart
c-Jun (Phospho-Ser63) Antibody
c-Jun (Phospho-Ser63) Antibody Datasheet  

  • Catalog Number:

    E011001-2

  • Amount:

    100μg/100μl

  • Reactivity:

    Human, Mouse

  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100

  • Swiss-Prot No.:

    P05412

  • Price:

    225$
  • Add to Cart
Rb Polyclonal Antibody
Rb Polyclonal Antibody Datasheet  

  • Catalog Number:

    E90003

  • Amount:

    100ul

  • Reactivity:

    H M

  • Applications:

    WB IHC

  • Swiss-Prot No.:

    P06400

  • Price:

    225$
  • Add to Cart
Elk-1 (Phospho-Ser383) Antibody
Elk-1 (Phospho-Ser383) Antibody Datasheet  

  • Catalog Number:

    E011004-1

  • Amount:

    50μg/50μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100

  • Swiss-Prot No.:

    P19419

  • Price:

    145$
  • Add to Cart
Elk-1 (Phospho-Ser383) Antibody
Elk-1 (Phospho-Ser383) Antibody Datasheet  

  • Catalog Number:

    E011004-2

  • Amount:

    100μg/100μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100

  • Swiss-Prot No.:

    P19419

  • Price:

    225$
  • Add to Cart

Catalog Number

  • Amount:100ul
  • Price:255$

Add to Cart

AbPromise

Contact us

BRG1 (4E11) Mouse mAb | EnoGene Biotech Co. Ltd