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eIF4A1 Mouse Monoclonal Antibody
Datasheet
Catalog Number:
E12-164Amount:
100μg/100μlClone Number:
M8Background:
The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA – this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression.Form of Antibody:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearSpecificity/Sensitivity:
Antibody can detects endogenous eIF4A1 protein.Reactivity:
H,R,MApplications:
WB: 1:1,000-3,000
- Western blot analysis of 1) 293T ,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue with E12-164 diluted at 1:2,000.
Price:
225$
Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using eIF4A1 (E12-164) Mouse mAb diluted at 1:200.
IF analysis of Hela with E12-164 (Left) and DAPI (Right) diluted at 1:100.
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