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PARP Mouse Monoclonal Antibody
Datasheet
Catalog Number:
E12-159Amount:
100μg/100μlClone Number:
M3Background:
Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.Form of Antibody:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearSpecificity/Sensitivity:
Antibody can detects endogenous PARP protein.Reactivity:
HApplications:
WB: 1:1,000-3,000
- Western blot analysis of 1) Hela, 2) 293T, 3) Jurkat with E12-159 diluted at 1:2,000.
Price:
225$
Immunohistochemical analysis of paraffin- embedded human Tonsil Tissue using PARP Mouse mAb diluted at 1:500.
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Catalog Number
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- Price:225$
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