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NFκB p65 Mouse Monoclonal Antibody
Datasheet   

  • Catalog Number:

    E12-138

  • Amount:

    100μg/100μl

  • Clone Number:

    5G6

  • Background:

    NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.

  • Form of Antibody:

    Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Storage/Stability:

    Store at -20℃/1 year. Do not aliquot the antibody.

  • Specificity/Sensitivity:

    NFκB p65 Mouse monoclonal antibody detects endogenous p65 proteins.

  • Reactivity:

    H, R, M

  • Price:

    225$

  • Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue with E12-138 diluted at 1:2000.
    1、Input: Hela Cell Lysate
    2、IP product: IP dilute 1:200
    Western blot analysis: primary antibody : E12-138 1:2,000
    Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000

  • IF analysis of Hela with E12-138(Left) and DAPI (Right) diluted at 1:100.

  • Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using NFkB p65 Mouse mAb diluted at

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Catalog Number

  • Amount:100μg/100μl
  • Price:225$

Add to Cart

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