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Histone H3(tri methyl K9) Mouse Monoclonal Antibody
Datasheet   

  • Catalog Number:

    E12-136

  • Amount:

    100μg/100μl

  • Clone Number:

    4H8

  • Background:

    Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

  • Form of Antibody:

    Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Storage/Stability:

    Store at -20℃/1 year. Do not aliquot the antibody.

  • Specificity/Sensitivity:

    The Histone H3(tri methyl K9) antibody can detects endogenous Histone H3(tri methyl K9) protein

  • Reactivity:

    H, R, M

  • Applications:

    WB: 1:1,000-3,000 IP:1:200

  • Western blot analysis of Hela with E12-136 diluted at
    1) 1:2,000
    2) 1:5,000

  • Price:

    225$

  • Western blot analysis of 1) Rat Testis Tissue, 2) Raw264.7 with E12-136 diluted at 1:2000.
    1、Input: Hela Cell Lysate
    2、IP product: IP dilute 1:200
    Western blot analysis: primary antibody : E12-136 1:1,000
    Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10,000

  • Immunohistochemical analysis of paraffin-embedded Human Colon using Histone H3(tri methyl K9) Mouse mAb diluted at 1:500.

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Catalog Number

  • Amount:100μg/100μl
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