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Caspase 9 Mouse Monoclonal Antibody
Datasheet
Catalog Number:
E12-102Amount:
100μg/100μlClone Number:
TDY3-20Background:
Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).Form of Antibody:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 year. Do not aliquot the antibody.Specificity/Sensitivity:
Antibody can detects endogenous Caspase 9 protein.Reactivity:
H, R, MApplications:
WB: 1:1,000-5,000 IP:1:200
- Western blot analysis of Hela with Caspase 9 (E12-3-20) Mouse mAb diluted at
1）1:2,000
2）1:5,000 Price:
225$
- Western blot analysis of 1）Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart with Caspase 9 (E12-3-20) Mouse mAb diluted at 1:3,000
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : E12-102 1:1,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000 
Western blot analysis of 1）Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart with Caspase 9 (3-20) Mouse mAb diluted at 1:3,000.
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