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NFκB p65 Mouse Monoclonal Antibody
Datasheet
Catalog Number:
E12-076Amount:
100μg/100μlClone Number:
14H2Background:
NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.Form of Antibody:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearSpecificity/Sensitivity:
NFκB p65 Mouse monoclonal antibody detects endogenous p65 proteins (~65KD ).Reactivity:
Human, Mouse, RatApplications:
WB: 1:1,000-3,000 IHC: 1:200
- Western blot analysis of Hela (lane 1), 293T(lane 2) and HepG (lane 3) with NFκB p65 mAb diluted at 1:2,000.
Western blot analysis of Mouse Heart (lane 1), Rat Heart (lane 2), Rat Liver (lane 3) and Rat Spleen (lane 4) with NFκB p65 mAb diluted at 1:3,000. 
- IHC staining of mouse hippocampus tissue with NFκB p65 mouse mAb(14H2) diluted at 1:200.
Price:
255$
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Catalog Number
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- Price:255$
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