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Lamin B1 Mouse Monoclonal Antibody (7C11)
Datasheet
Catalog Number:
E12-049Amount:
100ulClone Number:
7C11Background:
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins,Form of Antibody:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 year. Do not aliquot the antibody.Specificity/Sensitivity:
The α-tubulin antibody can detects endogenousα-tubulin proteinReactivity:
H, R, MApplications:
WB: 1:2,000-5,000 IP:1:200
- Western blot analysis of 1) HepG2, 2) 293T, 3) Mouse Brain Tissue, 4) Rat Brain Tissue with E12-049 diluted at 1:5,000.
1、Input: Mouse Brain Tissue Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : E12-049 1:5,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000
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