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PCNA Mouse Monoclonal Antibody
Datasheet
Catalog Number:
E12-044Amount:
100μg/100μlClone Number:
12D10Background:
PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.Form of Antibody:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearSpecificity/Sensitivity:
Antibody can detect endogenous PCNA protein.Applications:
WB: 1:5,000 IHC: 1:200
- Western blot analysis of Hela (lane 1), Rat brain (lane 2), NIH 3T3 (lane 3), 293T (lane 4) with PCNA mouse mAb (12D10) diluted at 1:5000.
IHC staining of Human breast cancer tissue paraffin- embedded with PCNA mouse mAb (12D10) diluted at 1:200. Price:
255$
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Catalog Number
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- Price:255$
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