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Mnk1 (Phospho-Thr385) Antibody
Datasheet
Catalog Number:
E11-8376AAmount:
100μgSwiss-Prot No.:
Q9BUB5Other Names:
EC 2.7.11.1; kinase Mnk1; Map kinase interacting kinase; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine kinase 1; MKNK1All Sites:
Human: Thr385; Mouse: Thr344; Rat: Thr332Storage/Stability:
Store at -20℃/1 yearForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Mnk1 around the phosphorylation site of threonine 385 (L-P-TP-P-Q).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
Mnk1 (Phospho-Thr385) antibody detects endogenous levels of Mnk1 only when phosphorylated at threonine 385.Reactivity:
Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000References:
Fukunaga R., EMBO J. 16:1921-1933(1997).
Knauf U., Mol. Cell. Biol. 21:5500-5511(2001).
O'Loghlen A., Exp. Cell Res. 299-343-355(2004).
- P-peptide - +
- Immunohistochemistry analysis of paraffin-embedded human brain tissue using Mnk1 (Phospho-Thr385) antibody.
Western blot analysis of extracts from COLO cells, treated with PMA (125ng/ml, 30mins), using Mnk1 (Phospho-Thr385) antibody. Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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