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GRK2 (Phospho-Ser685) Antibody
Datasheet   

  • Catalog Number:

    E11-8278A

  • Amount:

    100μg

  • Swiss-Prot No.:

    P25098

  • Other Names:

    ADRBK1; ARBK1; BARK; BARK1; beta-adrenergic receptor kinase 1; beta-ARK-1; EC 2.7.11.15; G protein receptor kinase 2; G- protein coupled receptor kinase 2; kinase GRK2

  • All Sites:

    Human: Ser685; Mouse: Ser685; Rat: Ser685

  • Storage/Stability:

    Store at -20℃/1 year

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human GRK2 around the phosphorylation site of serine 685 (R-G-SP-A-N).

  • Purification:

    The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    GRK2 (Phospho-Ser685) antibody detects endogenous levels of GRK2 only when phosphorylated at serine 685.

  • Reactivity:

    Human (Identities = 100%, Positives = 100%); Mouse (Identities = 91%, Positives = 100%);Rat (Identities = 91%, Positives = 100%)

  • Applications:

    WB: 1:500~1:1000 ELISA: 1:1000

  • References:

    Benovic J.L., FEBS Lett. 283:122-126(1991).
    Chuang T.T., J. Biol. Chem. 267:6886-6892(1992).
    Penn R.B., J. Biol. Chem. 269:14924-14930(1994).

  • Western blot analysis of extracts from HT-29 cells, treated with insulin (0.01U/ml, 15mins), using GRK2 (Phospho-Ser685) antibody.

  • Price:

    225$

Add to Cart

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Catalog Number

  • Amount:100μg
  • Price:225$

Add to Cart

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