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C-RAF (Phospho-Thr269) Antibody
Datasheet   

  • Catalog Number:

    E11-8177A
  • Amount:

    100μg
  • Swiss-Prot No.:

    P04049
  • Other Names:

    C-RAF; C-Raf; CRAF; EC 2.7.11.1; kinase Raf1; Protein kinase raf 1; RAF proto-oncogene serine/threonine-protein kinase; Raf proto-oncogene serine/threonine-protein kinase; RAF-1; Raf-1; RAF1; Similar to murine leukemia viral (V-raf-1) oncogene homolog 1
  • All Sites:

    Human: Thr269; Mouse: Thr269; Rat: Thr269
  • Storage/Stability:

    Store at -20℃/1 year
  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human C-RAF around the phosphorylation site of threonine 269 (S-T-TP-L-P).
  • Purification:

    The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Specificity/Sensitivity:

    C-RAF (Phospho-Thr269) antibody detects endogenous levels of C-RAF only when phosphorylated at threonine 269.
  • Reactivity:

    Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 92%);Rat (Identities = 100%, Positives = 100%)
  • Applications:

    IHC: 1:50~1:100 ELISA: 1:40000
  • References:

    The MGC Project Team; Genome Res. 14:2121-2127(2004).
    Olsen J.V., Cell 127:635-648(2006).
    Mott H.R., Proc. Natl. Acad. Sci. U.S.A. 93:8312-8317(1996).
  • P-peptide - +
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using C-RAF (Phospho-Thr269) antibody.
    C-RAF (Phospho-Thr269) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.
  • Price:

    225$
  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg
  • Price:225$

Add to Cart

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C-RAF (Phospho-Thr269) Antibody | EnoGene Biotech Co. Ltd