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MAP2K1 (Phospho-Thr386) Antibody
Datasheet   

  • Catalog Number:

    E11-8135A

  • Amount:

    100μg

  • Swiss-Prot No.:

    Q02750

  • Other Names:

    Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK1; MP2K1; PRKMK1; kinase MEK1

  • All Sites:

    Human: Thr386; Mouse: Thr386; Rat: Thr386

  • Storage/Stability:

    Store at -20℃/1 year

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human MAP2K1 around the phosphorylation site of threonine 386 (P-S-TP-P-T).

  • Purification:

    The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    MAP2K1 (Phospho-Thr386) antibody detects endogenous levels of MAP2K1 only when phosphorylated at threonine 386.

  • Reactivity:

    Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

  • Applications:

    WB: 1:500~1:1000 ELISA: 1:10000

  • References:

    Seger R., J. Biol. Chem. 267:25628-25631(1992).
    Zheng C.-F., J. Biol. Chem. 268:11435-11439(1993).
    Zheng C.-F., EMBO J. 13:1123-1131(1994).

  • Western blot analysis of extracts from A549 cells and RAW264.7 cells treated with UV, and NIH/3T3 cells treated with EGF, using MAP2K1 (Phospho-Thr386) antibody.

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg
  • Price:225$

Add to Cart

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