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BLK (Phospho-Tyr501) Antibody
Datasheet
Catalog Number:
E11-8081AAmount:
100μgSwiss-Prot No.:
P51451Other Names:
B lymphocyte kinase; EC 2.7.10.2; kinase Blk; p55-BLK; Tyrosine-protein kinase BLKAll Sites:
Human: Tyr501; Mouse: Tyr495; Rat: Tyr495Storage/Stability:
Store at -20℃/1 yearForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human BLK around the phosphorylation site of tyrosine 501 (R-Q-YP-E-L).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
BLK (Phospho-Tyr501) antibody detects endogenous levels of BLK only when phosphorylated at tyrosine 501.Reactivity:
Human (Identities = 100%, Positives = 100%); Mouse (Identities = 91%, Positives = 91%);Rat (Identities = 91%, Positives = 91%)Applications:
IHC: 1:50~1:100 ELISA: 1:20000References:
Islam K.B., J. Immunol. 154:1265-1272(1995).
Drebin J.A.,Oncogene 10:477-486(1995).
Greenman C.,Nature 446:153-158(2007).
- P-peptide - +
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using BLK (Phospho-Tyr501) antibody.
BLK (Phospho-Tyr501) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure. Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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