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AKT1/3 (Phospho-Tyr437/434) Antibody
Datasheet   

  • Catalog Number:

    E11-8076A

  • Amount:

    100μg

  • Swiss-Prot No.:

    P31749/Q9Y243

  • Other Names:

    EC 2.7.11.1; RAC-PK-alpha; Protein kinase B; PKB; C-AKT

  • All Sites:

    Human: Tyr437/434; Mouse: Tyr437/434; Rat: Tyr437/434

  • Storage/Stability:

    Store at -20℃/1 year

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human AKT1/3 around the phosphorylation site of tyrosine 437 (T-R-YP-F-D).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    AKT1/3 (Phospho-Tyr437/434) antibody detects endogenous levels of AKT1/3 only when phosphorylated at tyrosine 437.

  • Reactivity:

    Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

  • References:

    Jones P.F., Proc. Natl. Acad. Sci. U.S.A. 88:4171-4175(1991).
    Matsubara A., Diabetologia 44:910-913(2001).
    Coffer P.J., Eur. J. Biochem. 201:475-481(1991).

  • P-peptide - +

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using AKT1/3 (Phospho-Tyr437/434) antibody.
    Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using AKT1/3 (Phospho-Tyr437/434) antibody.

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg
  • Price:225$

Add to Cart

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AKT1/3 (Phospho-Tyr437/434) Antibody | EnoGene Biotech Co. Ltd