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ZAP-70 (Phospho-Tyr319) Antibody
Datasheet   

  • Catalog Number:

    E11-7255A

  • Amount:

    100μg/100μl

  • Swiss-Prot No.:

    P43403

  • Altername:

    70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70, ZAP-70, ZAP70, kinase ZAP70

  • All Sites:

    Human: Tyr319; Mouse: Tyr318; Rat: Tyr314

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Storage/Stability:

    Store at -20℃/1 year

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human Zap-70 around the phosphorylation site of tyrosine 319 (S-P-YP-S-D).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    Zap-70 (Phospho-Tyr319) antibody detects endogenous levels of Zap-70 only when phosphorylated at tyrosine 319. This antibody may cross-react with Syk when phosphorylated at equivalent sites.

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 ELISA: 1:1000

  • References:

    Salomon AR, et al. (2003) Proc Natl Acad Sci USA; 100(2): 443-448.
    Ku GM, et al. (2001) EMBO J; 20(3): 457-465.
    Tang J, et al. (1999) Proc Natl Acad Sci USA; 96(17): 9775-9780.
    Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774.
    Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844.

  • Western blot analysis of extracts from Jurkat cells,using Zap-70 (Ab-319) antibody (Line1 and 2) and Zap-70 (Phospho-Tyr319) antibody (Line 3 and 4).

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg/100μl
  • Price:225$

Add to Cart

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