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VAV1 (Phospho-Tyr174) Antibody
Datasheet
Catalog Number:
E11-7251AAmount:
100μg/100μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Vav around the phosphorylation site of tyrosine 174 (E-I-YP-E-D).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
Vav (phospho-Tyr174) antibody detects endogenous levels of Vav only when phosphorylated at tyrosine 174.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 ELISA: 1:10000Swiss-Prot No.:
P15498References:
Amarasinghe GK, et al. (2005) Biochemistry; 44(46): 15257-68. Charvet C, et al. (2005) J Biol Chem; 280(15): 15289-99. Miura-Shimura Y, et al. (2003) J Biol Chem; 278(40): 38495-504. Kuhne MR, et al. (2000) J Biol Chem; 275(3): 2185-90.
- Western blot analysis of the extracts from Jurkat cells using Vav (phospho-Tyr174) antibody.
Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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