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SEK1/MKK4 (Phospho-Ser80) Antibody
Datasheet
Catalog Number:
E11-7215AAmount:
100μg/100μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human SEK1/MKK4 around the phosphorylation site of serine 80 (T-H-SP-I-E).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
SEK1/MKK4 (phospho-Ser80) antibody detects endogenous levels of SEK1/MKK4 only when phosphorylated at serine 80.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IHC:1:50~1:100 ELISA: 1:5000Swiss-Prot No.:
P45985References:
Park HS, et al. (2002) J Biol Chem; 277(4): 2573-8.
- Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using SEK1/MKK4 (Phospho-Ser80) Antibody. Right: The same antibody preincubated with synthesized phosphopeptide.
- Western blot analysis of extracts from NIH/3T3 cells using SEK1/MKK4 (phospho-Ser80) antibody.
Price:
225$
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