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PKR (Phospho-Thr446) Antibody
Datasheet
Catalog Number:
E11-7198AAmount:
100μg/100μlSwiss-Prot No.:
P19525Altername:
EIF2AK2, Interferon-induced double-stranded RNA-activated protein kinaseAll Sites:
Human: Thr446; Mouse: Thr409Form of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PKR around the phosphorylation site of threonine 446 (K-R-TP-R-S).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
PKR (phospho-Thr446) antibody detects endogenous levels of PKR only when phosphorylated at threonine 446.Reactivity:
Human, MouseApplications:
IHC: 1:50~1:100 ELISA: 1:10000References:
Abujiang Pataer, et,al. (2002) Cancer Res ; 62: 2239.
K. D. Ryman, et,al. (2005) J. Virol ; 79: 1487-1499.
Susana Guerra, et,al. (2006) J. Biol. Chem ; 281: 18734-18745.
- Western blot analysis of extracts from K562 cells untreated or treated with Starvation (24 hours), using PKR (Ab-446) antibody (Line 1 and 2) and PKR (phospho-Thr446) antibody (Line 3 and 4).
Price:
225$
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