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NF-κB p65 (Phospho-Ser311) Antibody
Datasheet
Catalog Number:
E11-7163AAmount:
100μg/100μlSwiss-Prot No.:
Q04206Altername:
NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65, p65All Sites:
Human: Ser311; Mouse: Ser311; Rat: Ser311Form of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p65 around the phosphorylation site of serine 311 (F-K-SP-I-M).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
NF-κB p65 (phospho-Ser311) antibody detects endogenous levels of NF-κB p65 only when phosphorylated at serine 311.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:40000References:
Moon Kyoo Jang, J. Biol. Chem., Jun 2001; 276: 20005 - 20010.
Lluís Espinosa, Mol. Biol. Cell, Feb 2003; 14: 491.
Srinivas J. Kammanadiminti, Infect. Immun., Apr 2007; 75: 1765 - 1770.
D. Gregory, J. Virol., Dec 2004; 78: 13582 - 13590.
- P-peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p65 (phospho-Ser311) antibody. 
- Western blot analysis of extracts from 293 cells treated with TNF-α (20ng/ml, 30mins), using NF-κB p65 (phospho-Ser311) antibody (Line 1 and 2).
Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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