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MEK1 (Phospho-Thr291) Antibody
Datasheet   

  • Catalog Number:

    E11-7148A

  • Amount:

    100μg/100μl

  • Swiss-Prot No.:

    Q02750

  • Altername:

    MAP2K1, Dual specificity mitogen-activated protein kinase kinase 1

  • All Sites:

    Human: Thr 291; Mouse: Thr291; Rat: Thr291

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Storage/Stability:

    Store at -20℃/1 year

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of threonine 291(P-R-TP-P-G).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    MEK1 (phospho-Thr291) antibody detects endogenous levels of MEK1 only when phosphorylated at threonine 291.

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 ELISA: 1:10000

  • References:

    Kevin D. Burroughs, et,al. (2003) Mol. Cancer Res; 1: 312.
    Michael J. Piatelli, et,al. (2002) J. Biol. Chem; 277: 12144-12150.
    Margaret M. Morgan, et,al. (2001) J. Immunol; 167: 5708.
    Herbert Schramek, et,al. (2003) Am J Physiol Cell Physiol; 285: C652-C661.

  • Western blot analysis of extracts from K562/A431 cells untreated or treated with Serum (10%, 30mins), using MEK1 (Ab-291) antibody (Line 1 and 2) and MEK1 (phospho-Thr291) antibody (Line 3 and 4).

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg/100μl
  • Price:225$

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