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MEK1/2 (Phospho-Ser221) Antibody
Datasheet   

  • Catalog Number:

    E11-7147A

  • Amount:

    100μg/100μl

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Storage/Stability:

    Store at -20℃/1 year

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of serine 221 (A-N-SP-F-V).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    MEK1 (phospho-Ser221) antibody detects endogenous levels of MEK1 only when phosphorylated at serine 221.

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100
    ELISA:1:20000

  • Swiss-Prot No.:

    Q02750

  • References:

    Zebisch A, et al. (2006) Cancer Res; 66(7): 3401-8.
    Luciano BS, et al.(2006)J Biol Chem; 279(50): 52117-23.
    Wang X, et al. (2003) Oncogene; 22(1): 109-16.
    Gopalbhai K, et al. (2003) J Biol Chem; 278(10): 8118-25.
    Ling MT, et al. (2002)Oncogene; 21(55): 8498-505.

  • P-Peptide - +
    Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue using MEK1 (phospho- Ser221) antibody.

  • Western blot analysis of extracts from Jurkat cells, using MEK1 (Ab-221) antibody (Line 1 and 2) and MEK1 (phospho-Ser221) antibody (Line 3 and 4).

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg/100μl
  • Price:225$

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MEK1/2 (Phospho-Ser221) Antibody | EnoGene Biotech Co. Ltd