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IRS-1 (Phospho-Ser312) Antibody
Datasheet   

  • Catalog Number:

    E11-7121A

  • Amount:

    100μg

  • Swiss-Prot No.:

    P35568

  • Other Names:

    Insulin receptor substrate-1; IRS-1; IRS1

  • All Sites:

    Human: Ser24; Mouse: Ser24; Rat: Ser24

  • Storage/Stability:

    Store at -20℃/1 year

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 312 (A-T-SP-P-A).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    IRS-1 (Phospho-Ser312) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 312.

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

  • References:

    Araki E., Diabetes 42:1041-1054(1993).
    Nishiyama M., Biochem. Biophys. Res. Commun. 183:280-285(1992).
    Smith L.K., Biochem. Biophys. Res. Commun. 196:767-772(1993).

  • Peptide - +
    Western blot analysis of extracts from HT-29 cells, using IRS-1 (Phospho-Ser312) antibody.
    Immunofluorescence analysis of HeLa cells, using IRS-1 (Phospho-Ser312) antibody.

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

  • Western blot analysis of extracts from HT-29 cells, using IRS-1 (Phospho-Ser312) antibody.

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Catalog Number

  • Amount:100μg
  • Price:225$

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