Other Names Insulin receptor substrate-1; IRS-1; IRS1
All Sites Human: Ser24; Mouse: Ser24; Rat: Ser24
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 312 (A-T-SP-P-A).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity IRS-1 (Phospho-Ser312) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 312.
ReferencesAraki E., Diabetes 42:1041-1054(1993).
Nishiyama M., Biochem. Biophys. Res. Commun. 183:280-285(1992).
Smith L.K., Biochem. Biophys. Res. Commun. 196:767-772(1993).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells