Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human IKKα around the phosphorylation site of Threonine 23(L-G-TP-G-G).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityIKKα(Phospho-Thr23) Antibody detects endogenous levels of IKKα only when phosphorylated at Threonine 23.
ReferencesYuan ZQ, et al.(2002)J Biol Chem; 277(33): 29973-82.
Ozes ON, et al. (1999)Nature; 401(6748): 82-5.