HSP27 (Phospho-Ser82) Antibody

Name: HSP27 (Phospho-Ser82) Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E11-7112A
Price: 225 USD
Availability: InStock

E11-7112ACite ID: EN0025536Datasheet COA MSDS

ReactivityHuman

ApplicationsWB: 1:500~1:1000 ELISA: 1:4000

Swiss-Prot No.P04792

$225/ 100μg/100μl

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (1)Documents

All Names

AlternameHspB1; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; Estrogen-regulated 24 kDa protein; 28 kDa heat shock protein

All SitesHuman: Ser82

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/StabilityStore at -20℃/1 year

ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine 82 (Q-L-SP-S-G).

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityHSP27 (Phospho-Ser82) antibody detects endogenous levels of HSP27 only when phosphorylated at serine 82.

ReferencesSita Somara, et al. (2004) Am J Physiol Cell Physiol; 286: C1290-C1301.
Heike Döppler, et al. (2005) J. Biol. Chem; 280: 15013-15019.
Khalil N. Bitar, et al. (2002) Am J Physiol Gastrointest Liver Physiol; 282: 894.
J Cairns, et al. (1994) J. Biol. Chem; 269: 9176-9183.

Price225$

Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

Western blot analysis of extracts from Hela cells, untreated or treated with Ca 2+ , using HSP27 (Ab-82) antibody (Line 1 and 2) and HSP27 (Phospho- Ser82) antibody (Line 3 and 4).

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Certificate of Analysis (PDF)🧪MSDS
Material safety data sheet (PDF)

Conjugated Antibody

This product is also available conjugated. Format: E11-7112A-Conjugate. Price: $485 / Size: 100μl.

E11-7112A-FITC$485 / 100μlE11-7112A-Biotin$485 / 100μlE11-7112A-HRP$485 / 100μlE11-7112A-AP$485 / 100μlE11-7112A-AF350$485 / 100μlE11-7112A-AF405M$485 / 100μlE11-7112A-AF488$485 / 100μlE11-7112A-AF514$485 / 100μlE11-7112A-AF532$485 / 100μlE11-7112A-AF555$485 / 100μlE11-7112A-AF568$485 / 100μlE11-7112A-AF405S$485 / 100μlE11-7112A-AF405L$485 / 100μlE11-7112A-AF546$485 / 100μlE11-7112A-AF594$485 / 100μlE11-7112A-AF610$485 / 100μlE11-7112A-AF635$485 / 100μlE11-7112A-AF647$485 / 100μlE11-7112A-AF680$485 / 100μlE11-7112A-AF700$485 / 100μlE11-7112A-AF750$485 / 100μlE11-7112A-AF790$485 / 100μlE11-7112A-APC$485 / 100μlE11-7112A-PE$485 / 100μlE11-7112A-Cy3$485 / 100μlE11-7112A-Cy5$485 / 100μlE11-7112A-Cy5.5$485 / 100μlE11-7112A-Cy7$485 / 100μlE11-7112A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.