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Primary Antibodies

HSP27 (Ab-78) Antibody

E11-7111BCite ID: EN0025535DatasheetCOAMSDS
Human
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000
P04792
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameHspB1; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; Estrogen-regulated 24 kDa protein; 28 kDa heat shock protein

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine 78 (A-L-SP-R-Q).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificty Sensitivity

Specificity/SensitivityHSP27 (Ab-78) antibody detects endogenous levels of total HSP27 protein.
ReferencesJ Landry, et al. (1992) J. Biol. Chem; 267: 794-803.
Sita Somara and Khalil N. Bitar Am J Physiol, (2004) Cell Physiol; 286: C1290-C1301.
Elke Butt, et al. (2001) J. Biol. Chem; 276: 7108-7113.
Rainer Benndorf , et al. (2001) J. Biol. Chem; 276: 26753-26761.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 antibody.
Western blot analysis of extracts from Hela cells, untreated (Ab-78) or treated with Ca 2+ , using HSP27 (Ab-78) antibody (Line 1 and 2) and HSP27 (Phospho-Ser78) antibody (Line 3 and 4).
Western blot analysis of extracts from Hela cells, untreated (Ab-78) or treated with Ca 2+ , using HSP27 (Ab-78) antibody (Line 1 and 2) and HSP27 (Phospho-Ser78) antibody (Line 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E11-7111B-Conjugate. Price: $485 / Size: 100μl.

E11-7111B-FITC$485 / 100μlE11-7111B-Biotin$485 / 100μlE11-7111B-HRP$485 / 100μlE11-7111B-AP$485 / 100μlE11-7111B-AF350$485 / 100μlE11-7111B-AF405M$485 / 100μlE11-7111B-AF488$485 / 100μlE11-7111B-AF514$485 / 100μlE11-7111B-AF532$485 / 100μlE11-7111B-AF555$485 / 100μlE11-7111B-AF568$485 / 100μlE11-7111B-AF405S$485 / 100μlE11-7111B-AF405L$485 / 100μlE11-7111B-AF546$485 / 100μlE11-7111B-AF594$485 / 100μlE11-7111B-AF610$485 / 100μlE11-7111B-AF635$485 / 100μlE11-7111B-AF647$485 / 100μlE11-7111B-AF680$485 / 100μlE11-7111B-AF700$485 / 100μlE11-7111B-AF750$485 / 100μlE11-7111B-AF790$485 / 100μlE11-7111B-APC$485 / 100μlE11-7111B-PE$485 / 100μlE11-7111B-Cy3$485 / 100μlE11-7111B-Cy5$485 / 100μlE11-7111B-Cy5.5$485 / 100μlE11-7111B-Cy7$485 / 100μlE11-7111B-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.