All Names
AlternameHspB1; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; Estrogen-regulated 24 kDa protein; 28 kDa heat shock protein
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine 78 (A-L-SP-R-Q).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityHSP27 (Phospho-Ser78) antibody detects endogenous levels of HSP27 only when phosphorylated at serine 78.
ReferencesJ Landry, et al. (1992) J. Biol. Chem; 267: 794-803.
Sita Somara and Khalil N. Bitar Am J Physiol, (2004) Cell Physiol; 286: C1290-C1301.
Elke Butt, et al. (2001) J. Biol. Chem; 276: 7108-7113.
Rainer Benndorf, et al. (2001) J. Biol. Chem; 276: 26753-26761.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells