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Primary Antibodies

HSP27 (Phospho-Ser78) Antibody

E11-7111ACite ID: EN0025534DatasheetCOAMSDS
Human
WB: 1:500~1:1000 ELISA: 1:20000
P04792
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameHspB1; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; Estrogen-regulated 24 kDa protein; 28 kDa heat shock protein
All SitesHuman: Ser78

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine 78 (A-L-SP-R-Q).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityHSP27 (Phospho-Ser78) antibody detects endogenous levels of HSP27 only when phosphorylated at serine 78.
ReferencesJ Landry, et al. (1992) J. Biol. Chem; 267: 794-803.
Sita Somara and Khalil N. Bitar Am J Physiol, (2004) Cell Physiol; 286: C1290-C1301.
Elke Butt, et al. (2001) J. Biol. Chem; 276: 7108-7113.
Rainer Benndorf, et al. (2001) J. Biol. Chem; 276: 26753-26761.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from Hela cells, untreated or treated with Ca 2+ , using HSP27 (Ab-78) antibody (Line 1 and 2) and HSP27 (Phospho- Ser78) antibody (Line 3 and 4).
Western blot analysis of extracts from Hela cells, untreated or treated with Ca 2+ , using HSP27 (Ab-78) antibody (Line 1 and 2) and HSP27 (Phospho- Ser78) antibody (Line 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E11-7111A-Conjugate. Price: $485 / Size: 100μl.

E11-7111A-FITC$485 / 100μlE11-7111A-Biotin$485 / 100μlE11-7111A-HRP$485 / 100μlE11-7111A-AP$485 / 100μlE11-7111A-AF350$485 / 100μlE11-7111A-AF405M$485 / 100μlE11-7111A-AF488$485 / 100μlE11-7111A-AF514$485 / 100μlE11-7111A-AF532$485 / 100μlE11-7111A-AF555$485 / 100μlE11-7111A-AF568$485 / 100μlE11-7111A-AF405S$485 / 100μlE11-7111A-AF405L$485 / 100μlE11-7111A-AF546$485 / 100μlE11-7111A-AF594$485 / 100μlE11-7111A-AF610$485 / 100μlE11-7111A-AF635$485 / 100μlE11-7111A-AF647$485 / 100μlE11-7111A-AF680$485 / 100μlE11-7111A-AF700$485 / 100μlE11-7111A-AF750$485 / 100μlE11-7111A-AF790$485 / 100μlE11-7111A-APC$485 / 100μlE11-7111A-PE$485 / 100μlE11-7111A-Cy3$485 / 100μlE11-7111A-Cy5$485 / 100μlE11-7111A-Cy5.5$485 / 100μlE11-7111A-Cy7$485 / 100μlE11-7111A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.