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HistoneH2A.X(Phospho-Ser139)Antibody
Datasheet
Catalog Number:
E11-7106AAmount:
100μg/100μlSwiss-Prot No.:
P16104Altername:
H2a/xAll Sites:
Human: Ser139; Mouse: Ser139Form of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
Histone H2A.X (Phospho-Ser139) antibody detects endogenous levels of Histone H2A.X only when phosphorylated at serine 139.Reactivity:
Human, MouseApplications:
WB: 1:500~1:1000 ELISA: 1:10000References:
Alpna Tyagi, et al. (2005) Carcinogenesis; 26: 1978-1987.
James P, et al. (2002) Nucleic Acids Res; 30: 3698-3705.
Mariana Yaneva, et al. (2005) Nucleic Acids Res; 33: 5320-5330.
Jérôme Govin, et al. (2004) Eur. J. Biochem; 271: 3459-3469
- Western blot analysis of extracts from Hela cells, untreated or treated with UV, using Histone H2A.X (Ab-139) antibody (Line 1 and 2) and Histone H2A.X (Phospho-Ser139) antibody (Line 3 and 4).
Price:
225$
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