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HER2 (Ab-877) Antibody
Datasheet
Catalog Number:
E11-7105BAmount:
100μg/100μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human HER2 around the phosphorylation site of Tyrosine 877.Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Specificity/Sensitivity:
HER2 (Ab-877) Antibody detects endogenous levels of total HER2 protein.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000Swiss-Prot No.:
P04626References:
Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444.
Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857.
Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504.
Kwon, Y. K. et al. (1997) J. Neurosci. 17, 8293-8299.
Klapper, L. N. et al. (2000) Cancer Res. 60, 3384-3388.
- Immunohistochemical analysis of paraffin- embedded breast carcinoma, using HER2 (Ab-877) Antibody (#B7105). Left: Untreated; Right: Treated with synthesized peptide.
- Western blot analysis of extracts from MDA-MB-231 cells treated or untreated with EGF using HER2 (Ab-877) Antibody (#B7105) and HER2 (Phospho- Tyr877) Antibody (#11169). Line1:Using #B7105; Line2: Using #B7105 preincubated with synthesized peptide.
Price:
225$
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