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Primary Antibodies

HER2(Phospho-Tyr1221/Tyr1222)Antibody

E11-7103ACite ID: EN0025518DatasheetCOAMSDS
Human
WB: 1:500~1:1000 IHC: 1:50~1:100ELISA: 1:20000 IP: Various Dilution
P04626
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine1221/1222 (N-L-YP-YP -W).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityHER2 (phospho-Tyr1221/Tyr1222) antibody detects endogenous levels of HER2 only when phosphorylated at tyrosine 1221/1222.
ReferencesMarone R, et al. (2004) Nat Cell Biol; 6(6): 515-22.
Ren Z, et al. (2002) J Biol Chem; 277(41): 38486-93.
Price225$
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using HER2 (phospho-Tyr1221/Tyr1222) antibody.
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using HER2 (phospho-Tyr1221/Tyr1222) antibody.
Western blot analysis of extracts from SK-OV3 cells using HER2 (Ab-1221/1222) antibody (Line 1 and 2) and HER2 (phospho-Tyr1221/Tyr1222) antibody (Line 3 and 4).
Western blot analysis of extracts from SK-OV3 cells using HER2 (Ab-1221/1222) antibody (Line 1 and 2) and HER2 (phospho-Tyr1221/Tyr1222) antibody (Line 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E11-7103A-Conjugate. Price: $485 / Size: 100μl.

E11-7103A-FITC$485 / 100μlE11-7103A-Biotin$485 / 100μlE11-7103A-HRP$485 / 100μlE11-7103A-AP$485 / 100μlE11-7103A-AF350$485 / 100μlE11-7103A-AF405M$485 / 100μlE11-7103A-AF488$485 / 100μlE11-7103A-AF514$485 / 100μlE11-7103A-AF532$485 / 100μlE11-7103A-AF555$485 / 100μlE11-7103A-AF568$485 / 100μlE11-7103A-AF405S$485 / 100μlE11-7103A-AF405L$485 / 100μlE11-7103A-AF546$485 / 100μlE11-7103A-AF594$485 / 100μlE11-7103A-AF610$485 / 100μlE11-7103A-AF635$485 / 100μlE11-7103A-AF647$485 / 100μlE11-7103A-AF680$485 / 100μlE11-7103A-AF700$485 / 100μlE11-7103A-AF750$485 / 100μlE11-7103A-AF790$485 / 100μlE11-7103A-APC$485 / 100μlE11-7103A-PE$485 / 100μlE11-7103A-Cy3$485 / 100μlE11-7103A-Cy5$485 / 100μlE11-7103A-Cy5.5$485 / 100μlE11-7103A-Cy7$485 / 100μlE11-7103A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.