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Primary Antibodies

GSK3α(Phospho-Ser21) Antibody

E11-7097ACite ID: EN0025505DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000 IP: Various Dilution
P49840
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human GSK3α around the phosphorylation site of Serine 21.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityGSK3α(Phospho-Ser21) Antibody detects endogenous levels of GSK3α only when phosphorylated at Serine 21.
ReferencesBarry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Koivisto L, et al. (2003) J Cell Sci. 116(Pt 18): 3749-3760.
Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279.
Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.
Price225$
Immunohistochemical analysis of paraffin- embedded breast carcinoma. Left: Using GSK3α (Phospho-Ser21) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.
Immunohistochemical analysis of paraffin- embedded breast carcinoma. Left: Using GSK3α (Phospho-Ser21) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.
Western blot analysis of extracts from ovary cancer cells. Line1: Using GSK3α(Phospho-Ser21) Antibody; Line2: The same antibody preincubated with synthesized non-phospho- peptide; Line3: The same antibody preincubated with synthesized phosphopeptide.
Western blot analysis of extracts from ovary cancer cells. Line1: Using GSK3α(Phospho-Ser21) Antibody; Line2: The same antibody preincubated with synthesized non-phospho- peptide; Line3: The same antibody preincubated with synthesized phosphopeptide.

Conjugated Antibody

This product is also available conjugated. Format: E11-7097A-Conjugate. Price: $485 / Size: 100μl.

E11-7097A-FITC$485 / 100μlE11-7097A-Biotin$485 / 100μlE11-7097A-HRP$485 / 100μlE11-7097A-AP$485 / 100μlE11-7097A-AF350$485 / 100μlE11-7097A-AF405M$485 / 100μlE11-7097A-AF488$485 / 100μlE11-7097A-AF514$485 / 100μlE11-7097A-AF532$485 / 100μlE11-7097A-AF555$485 / 100μlE11-7097A-AF568$485 / 100μlE11-7097A-AF405S$485 / 100μlE11-7097A-AF405L$485 / 100μlE11-7097A-AF546$485 / 100μlE11-7097A-AF594$485 / 100μlE11-7097A-AF610$485 / 100μlE11-7097A-AF635$485 / 100μlE11-7097A-AF647$485 / 100μlE11-7097A-AF680$485 / 100μlE11-7097A-AF700$485 / 100μlE11-7097A-AF750$485 / 100μlE11-7097A-AF790$485 / 100μlE11-7097A-APC$485 / 100μlE11-7097A-PE$485 / 100μlE11-7097A-Cy3$485 / 100μlE11-7097A-Cy5$485 / 100μlE11-7097A-Cy5.5$485 / 100μlE11-7097A-Cy7$485 / 100μlE11-7097A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.