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Primary Antibodies

GAB1 (Phospho-Tyr627) Antibody

E11-7090ACite ID: EN0025491DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1:1000 ELISA: 1:10000
Q13480
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameGAB1, GRB2-associated-binding protein 1
All SitesHuman: Tyr627; Mouse: Tyr628

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Gab1 around the phosphorylation site of tyrosine 627 (V-E-YP-L-D).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityGab1(phospho-Tyr627) antibody detects endogenous levels of Gab1 only when phosphorylated at tyrosine 627.
ReferencesAnders Kallin, et,al. (2004) J. Biol. Chem; 279: 17897-17904.
Hideto Kameda, et,al. (2001) Cell Growth Differ; 12: 307.
Masaki Osawa, et,al. (2002) J. Cell Biol; 158: 773.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from HuvEc cells untreated or treated with EGF (200ng/ml, 10mins), using Gab1 (Ab-627) antibody (Line 1 and 2) and Gab1 (phospho-Tyr627) antibody (Line 3 and 4).
Western blot analysis of extracts from HuvEc cells untreated or treated with EGF (200ng/ml, 10mins), using Gab1 (Ab-627) antibody (Line 1 and 2) and Gab1 (phospho-Tyr627) antibody (Line 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E11-7090A-Conjugate. Price: $485 / Size: 100μl.

E11-7090A-FITC$485 / 100μlE11-7090A-Biotin$485 / 100μlE11-7090A-HRP$485 / 100μlE11-7090A-AP$485 / 100μlE11-7090A-AF350$485 / 100μlE11-7090A-AF405M$485 / 100μlE11-7090A-AF488$485 / 100μlE11-7090A-AF514$485 / 100μlE11-7090A-AF532$485 / 100μlE11-7090A-AF555$485 / 100μlE11-7090A-AF568$485 / 100μlE11-7090A-AF405S$485 / 100μlE11-7090A-AF405L$485 / 100μlE11-7090A-AF546$485 / 100μlE11-7090A-AF594$485 / 100μlE11-7090A-AF610$485 / 100μlE11-7090A-AF635$485 / 100μlE11-7090A-AF647$485 / 100μlE11-7090A-AF680$485 / 100μlE11-7090A-AF700$485 / 100μlE11-7090A-AF750$485 / 100μlE11-7090A-AF790$485 / 100μlE11-7090A-APC$485 / 100μlE11-7090A-PE$485 / 100μlE11-7090A-Cy3$485 / 100μlE11-7090A-Cy5$485 / 100μlE11-7090A-Cy5.5$485 / 100μlE11-7090A-Cy7$485 / 100μlE11-7090A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.