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FKHR (Phospho-Ser319) Antibody
Datasheet
Catalog Number:
E11-7086AAmount:
100μg/100μlSwiss-Prot No.:
Q12778Altername:
FKHR, FOXO1, Forkhead box protein O1A, Forkhead in rhabdomyosarcomaAll Sites:
Human: Ser319; Mouse: Ser316; Rat: Ser313Form of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human FKHR around the phosphorylation site of serine 319 (T-S-SP-N-A).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
FKHR (phospho-Ser319) antibody detects endogenous levels of FKHR only when phosphorylated at serine 319.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:10000References:
Rena G, et al. (2002) EMBO J 21(9): 2263-2271.
Woods YL, et al. (2001) Biochem J355(Pt 3): 597-607.
Rena G, et al. (2001) Biochem J 354(Pt 3): 605-612.
- P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR (phospho- Ser319) antibody . 
- Western blot analysis of extracts from 293 cells (10% serum-treated, 15min) and HeLa cells (EGF-treated, 200ng/ml, 15min), using FKHR (Ab-319) antibody (Line 1, 2 and 3) and FKHR (phospho-Ser319) antibody (Line 4 and 5).
Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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