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FAK (Phospho-Tyr397) Antibody
Datasheet
Catalog Number:
E11-7081AAmount:
100μgSwiss-Prot No.:
Q05397Other Names:
EC 2.7.10.2; FADK 1; FAK1; Focal adhesion kinase 1; pp125FAK; Protein- tyrosine kinase 2; PTK2Storage/Stability:
Store at -20℃/1 yearForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 397 (D-D-YP-A-E).Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
FAK (Phospho-Tyr397) antibody detects endogenous levels of FAK only when phosphorylated at tyrosine 397.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 ELISA: 1:5000References:
Whitney G.S., DNA Cell Biol. 12:823-830(1993).
Andre E., Biochem. Biophys. Res. Commun. 190:140-147(1993).
Lee S.-T., Oncogene 8:3403-3410(1993).
- Western blot analysis of extracts from 293cells, treated with EGF (200ng/ml, 30mins), using FAK (Phospho-Tyr397) antibody.
Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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