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Ezrin (Phospho-Tyr353) Antibody
Datasheet
Catalog Number:
E11-7080AAmount:
100μg/100μlSwiss-Prot No.:
P15311Altername:
Cytovillin, EZRI, VIL2, Villin 2, p81All Sites:
Human: Tyr353; Mouse: Tyr353Form of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 353 (Q-D-YP-E-E).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
Ezrin (phospho-Tyr353) antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 353.Reactivity:
Human, MouseApplications:
WB: 1:500~1:1000 ELISA: 1:20000References:
Zhao H, et al. (2004) Proc Natl Acad Sci U S A101 (25): 9485-90.
Wang Q, et al. (2003) J Biol Chem 278(48): 47731-43.
Gautreau A, et al. (1999) Proc Natl Acad Sci U S A: 96(13): 7300-5.
Crepaldi T, et al. (1997) J Cell Biol: 28; 138(2): 423-34.
- Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30mins), using Ezrin (Ab-353) antibody (Line 1 and 2) and Ezrin (phospho-Tyr353) antibody (Line 3 and 4).
Price:
225$
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