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Ezrin (Phospho-Thr566) Antibody
Datasheet   

  • Catalog Number:

    E11-7079A

  • Amount:

    100μg/100μl

  • Swiss-Prot No.:

    P15311

  • Altername:

    Cytovillin, EZRI, VIL2, Villin 2, p81

  • All Sites:

    Human: Thr566 or Thr567; Mouse:Thr566 or Thr567; Rat: Thr567

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Storage/Stability:

    Store at -20℃/1 year

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 566 (Y-K-TP-L-R).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    Ezrin (phospho-Tyr566) antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 566.

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 ELISA: 1:20000

  • References:

    Zhou R, et al. (2005) J Cell Sci: 118 (Pt 19): 4381-91
    Shiue H, et al.(2005) J Biol Chem: 280(2): 1688-95
    Zhao H, et al. (2004) Proc Natl Acad Sci U S A: 101(25): 9485-90
    Fievet BT, et al. (2004) J Cell Biol: 164(5): 653-9

  • Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30mins) using Ezrin (Ab-566) antibody (Line 1 and 2) and Ezrin (phospho-Tyr566) antibody (Line 3 and 4).

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg/100μl
  • Price:225$

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