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Primary Antibodies

Ezrin (Phospho-Thr566) Antibody

E11-7079ACite ID: EN0025468DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 ELISA: 1:20000
P15311
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameCytovillin, EZRI, VIL2, Villin 2, p81
All SitesHuman: Thr566 or Thr567; Mouse:Thr566 or Thr567; Rat: Thr567

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 566 (Y-K-TP-L-R).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityEzrin (phospho-Tyr566) antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 566.
ReferencesZhou R, et al. (2005) J Cell Sci: 118 (Pt 19): 4381-91
Shiue H, et al.(2005) J Biol Chem: 280(2): 1688-95
Zhao H, et al. (2004) Proc Natl Acad Sci U S A: 101(25): 9485-90
Fievet BT, et al. (2004) J Cell Biol: 164(5): 653-9
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30mins) using Ezrin (Ab-566) antibody (Line 1 and 2) and Ezrin (phospho-Tyr566) antibody (Line 3 and 4).
Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30mins) using Ezrin (Ab-566) antibody (Line 1 and 2) and Ezrin (phospho-Tyr566) antibody (Line 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E11-7079A-Conjugate. Price: $485 / Size: 100μl.

E11-7079A-FITC$485 / 100μlE11-7079A-Biotin$485 / 100μlE11-7079A-HRP$485 / 100μlE11-7079A-AP$485 / 100μlE11-7079A-AF350$485 / 100μlE11-7079A-AF405M$485 / 100μlE11-7079A-AF488$485 / 100μlE11-7079A-AF514$485 / 100μlE11-7079A-AF532$485 / 100μlE11-7079A-AF555$485 / 100μlE11-7079A-AF568$485 / 100μlE11-7079A-AF405S$485 / 100μlE11-7079A-AF405L$485 / 100μlE11-7079A-AF546$485 / 100μlE11-7079A-AF594$485 / 100μlE11-7079A-AF610$485 / 100μlE11-7079A-AF635$485 / 100μlE11-7079A-AF647$485 / 100μlE11-7079A-AF680$485 / 100μlE11-7079A-AF700$485 / 100μlE11-7079A-AF750$485 / 100μlE11-7079A-AF790$485 / 100μlE11-7079A-APC$485 / 100μlE11-7079A-PE$485 / 100μlE11-7079A-Cy3$485 / 100μlE11-7079A-Cy5$485 / 100μlE11-7079A-Cy5.5$485 / 100μlE11-7079A-Cy7$485 / 100μlE11-7079A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.