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p44/42 MAP Kinase (Phospho-Tyr204) Antibody
Datasheet   

  • Catalog Number:

    E11-7074A

  • Amount:

    100μg/100μl

  • Swiss-Prot No.:

    P27361

  • Altername:

    Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAPK 1, p44-ERK1, ERT2, p44-MAPK, Microtubule-associated protein 2 kinase

  • All Sites:

    Human: Tyr204; Mouse: Tyr204; Rat:Tyr204

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Storage/Stability:

    Store at -20℃/1 year

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of tyrosine 204 (T-E-YP-V-A).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    p44/42 MAP Kinase (Phospho-Tyr204) antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at tyrosine 204.

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 ELISA: 1:10000

  • References:

    Jun Tan, et al. (2000) J. Biol. Chem; 275: 37224.
    Tete Hannken, et al. (2000) J. Am. Soc. Nephrol; 11: 1387.
    Jun Tan, et al. (1999) J. Immunol; 163: 6614.
    Reinoud Gosens, et al. (2006) Am J Physiol Lung Cell Mol Physiol; 291: L523-L534.

  • Western blot analysis of extracts from Jurkat cells, untreated or PMA-treated (200 ng/ml, 15mins), using p44/42 MAP Kinase (Ab-204) antibody (Line 1 and 2) and p44/42 MAP Kinase (Phospho-Tyr204) antibody (Line 3 and 4).

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg/100μl
  • Price:225$

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p44/42 MAP Kinase (Phospho-Tyr204) Antibody | EnoGene Biotech Co. Ltd