Other Names cNOS; Constitutive NOS; EC 1.14.13.39; EC-NOS; ECNOS; Endothelial NOS; Nitric-oxide synthase, endothelial; NOS, type III; NOS3; NOSIII
All Sites Human: Thr494; Mouse: Thr494; Rat: Thr494
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of threonine 494 (K-K-TP-F-K).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity eNOS (Phospho-Thr494) antibody detects endogenous levels of eNOS only when phosphorylated at threonine 494.
ReferencesJanssens S.P., J. Biol. Chem. 267:14519-14522(1992).
Janssens S.P., J. Biol. Chem. 267:22694-22694(1992).
Marsden P.A., FEBS Lett. 307:287-293(1992).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells