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Primary Antibodies

eNOS (Phospho-Thr494) Antibody

E11-7072ACite ID: EN0025454DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 ELISA: 1:5000
P29474
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names cNOS; Constitutive NOS; EC 1.14.13.39; EC-NOS; ECNOS; Endothelial NOS; Nitric-oxide synthase, endothelial; NOS, type III; NOS3; NOSIII
All Sites Human: Thr494; Mouse: Thr494; Rat: Thr494
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of threonine 494 (K-K-TP-F-K).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity eNOS (Phospho-Thr494) antibody detects endogenous levels of eNOS only when phosphorylated at threonine 494.
ReferencesJanssens S.P., J. Biol. Chem. 267:14519-14522(1992).
Janssens S.P., J. Biol. Chem. 267:22694-22694(1992).
Marsden P.A., FEBS Lett. 307:287-293(1992).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from Jurkat cells and HepG2 cells, using eNOS (Phospho-Thr494) antibody.
Western blot analysis of extracts from Jurkat cells and HepG2 cells, using eNOS (Phospho-Thr494) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-7072A-Conjugate. Price: $485 / Size: 100μl.

E11-7072A-FITC$485 / 100μlE11-7072A-Biotin$485 / 100μlE11-7072A-HRP$485 / 100μlE11-7072A-AP$485 / 100μlE11-7072A-AF350$485 / 100μlE11-7072A-AF405M$485 / 100μlE11-7072A-AF488$485 / 100μlE11-7072A-AF514$485 / 100μlE11-7072A-AF532$485 / 100μlE11-7072A-AF555$485 / 100μlE11-7072A-AF568$485 / 100μlE11-7072A-AF405S$485 / 100μlE11-7072A-AF405L$485 / 100μlE11-7072A-AF546$485 / 100μlE11-7072A-AF594$485 / 100μlE11-7072A-AF610$485 / 100μlE11-7072A-AF635$485 / 100μlE11-7072A-AF647$485 / 100μlE11-7072A-AF680$485 / 100μlE11-7072A-AF700$485 / 100μlE11-7072A-AF750$485 / 100μlE11-7072A-AF790$485 / 100μlE11-7072A-APC$485 / 100μlE11-7072A-PE$485 / 100μlE11-7072A-Cy3$485 / 100μlE11-7072A-Cy5$485 / 100μlE11-7072A-Cy5.5$485 / 100μlE11-7072A-Cy7$485 / 100μlE11-7072A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.