All Names
AlternameConstitutive NOS, EC 1.14.13.39, EC-NOS, ECNOS, Endothelial NOS, NOS, type III, NOS3, NOSIII, Nitric-oxide synthase, endothelial, cNOS
All SitesHuman: Ser1176; Mouse: Ser1175; Rat: Ser1175
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of serine 1176 (T-Q-SP-F-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityeNOS (phospho-Ser1176) antibody detects endogenous levels of eNOS only when phosphorylated at serine 1176 .
ReferencesYong Chool Boo, Am J Physiol Heart Circ Physiol, Nov 2002; 283: 1819.
Christian Pott, Am J Physiol Cell Physiol, May 2006; 290: C1437 - C1445.
Monica Montagnani, J. Biol. Chem., Aug 2001; 276: 30392 - 30398.
Peter F. Mount, Am J Physiol Renal Physiol, Nov 2005; 289: F1103 - F1115.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells