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Primary Antibodies

eNOS (Phospho-Ser1176) Antibody

E11-7071ACite ID: EN0025452DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 ELISA: 1:20000
P29474
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameConstitutive NOS, EC 1.14.13.39, EC-NOS, ECNOS, Endothelial NOS, NOS, type III, NOS3, NOSIII, Nitric-oxide synthase, endothelial, cNOS
All SitesHuman: Ser1176; Mouse: Ser1175; Rat: Ser1175

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of serine 1176 (T-Q-SP-F-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityeNOS (phospho-Ser1176) antibody detects endogenous levels of eNOS only when phosphorylated at serine 1176 .
ReferencesYong Chool Boo, Am J Physiol Heart Circ Physiol, Nov 2002; 283: 1819.
Christian Pott, Am J Physiol Cell Physiol, May 2006; 290: C1437 - C1445.
Monica Montagnani, J. Biol. Chem., Aug 2001; 276: 30392 - 30398.
Peter F. Mount, Am J Physiol Renal Physiol, Nov 2005; 289: F1103 - F1115.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from Hela cells treated with Insulin (0.01U/ml, 15mins), using eNOS (phospho-Ser1176) antibody (Line 1 and 2). For Research Use Only
Western blot analysis of extracts from Hela cells treated with Insulin (0.01U/ml, 15mins), using eNOS (phospho-Ser1176) antibody (Line 1 and 2). For Research Use Only

Conjugated Antibody

This product is also available conjugated. Format: E11-7071A-Conjugate. Price: $485 / Size: 100μl.

E11-7071A-FITC$485 / 100μlE11-7071A-Biotin$485 / 100μlE11-7071A-HRP$485 / 100μlE11-7071A-AP$485 / 100μlE11-7071A-AF350$485 / 100μlE11-7071A-AF405M$485 / 100μlE11-7071A-AF488$485 / 100μlE11-7071A-AF514$485 / 100μlE11-7071A-AF532$485 / 100μlE11-7071A-AF555$485 / 100μlE11-7071A-AF568$485 / 100μlE11-7071A-AF405S$485 / 100μlE11-7071A-AF405L$485 / 100μlE11-7071A-AF546$485 / 100μlE11-7071A-AF594$485 / 100μlE11-7071A-AF610$485 / 100μlE11-7071A-AF635$485 / 100μlE11-7071A-AF647$485 / 100μlE11-7071A-AF680$485 / 100μlE11-7071A-AF700$485 / 100μlE11-7071A-AF750$485 / 100μlE11-7071A-AF790$485 / 100μlE11-7071A-APC$485 / 100μlE11-7071A-PE$485 / 100μlE11-7071A-Cy3$485 / 100μlE11-7071A-Cy5$485 / 100μlE11-7071A-Cy5.5$485 / 100μlE11-7071A-Cy7$485 / 100μlE11-7071A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.