Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Elk-1 around the phosphorylation site of Serine 383.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityElk-1 (Phospho-Ser383) Antibody detects endogenous levels of Elk-1 only when phosphorylated at Serine 383.
ReferencesXing J, et al. (1996) Science. 273(5277): 959-963.
Janknecht R, et al. (1993) EMBO J. 12(13): 5097-5104.
Marais R, et al. (1993) Cell 73: 381-393.
Kortenjann M, et al. (1994) Mol Cell Biol. 14: 4815-4824.
Cavigelli M, et al. (1995) EMBO J. 14: 5957-5964.