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Primary Antibodies

eIF4E (Phospho-Ser209) Antibody

E11-7067ACite ID: EN0025444DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000
P06730
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameEukaryotic translation initiation factor 4E, IF4E, eIF-4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein
All SitesHuman: Ser209; Mouse: Ser 209; Rat: Ser 209

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityelF4E (phospho-Ser209) antibody detects endogenous levels of elF4E only when phosphorylated at serine 209.
ReferencesLi BD, et al. (1998) Ann Surg; 227(5): 756-763.
Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520.
De Gregorio E, et al. (2001) RNA; 7(1): 106-113.
Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded Western human breast carcinoma tissue using elF4E (phospho- untreated Ser209) antibody. antibody antibody
Immunohistochemical analysis of paraffin-embedded Western human breast carcinoma tissue using elF4E (phospho- untreated Ser209) antibody. antibody antibody
blot analysis of extracts from NIH/3T3 cells or treated with FBS, using elF4E (Ab-209) (Line 1 and 2) and elF4E (Phospho- Ser209) (Line 3 and 4). Use Only
blot analysis of extracts from NIH/3T3 cells or treated with FBS, using elF4E (Ab-209) (Line 1 and 2) and elF4E (Phospho- Ser209) (Line 3 and 4). Use Only

Conjugated Antibody

This product is also available conjugated. Format: E11-7067A-Conjugate. Price: $485 / Size: 100μl.

E11-7067A-FITC$485 / 100μlE11-7067A-Biotin$485 / 100μlE11-7067A-HRP$485 / 100μlE11-7067A-AP$485 / 100μlE11-7067A-AF350$485 / 100μlE11-7067A-AF405M$485 / 100μlE11-7067A-AF488$485 / 100μlE11-7067A-AF514$485 / 100μlE11-7067A-AF532$485 / 100μlE11-7067A-AF555$485 / 100μlE11-7067A-AF568$485 / 100μlE11-7067A-AF405S$485 / 100μlE11-7067A-AF405L$485 / 100μlE11-7067A-AF546$485 / 100μlE11-7067A-AF594$485 / 100μlE11-7067A-AF610$485 / 100μlE11-7067A-AF635$485 / 100μlE11-7067A-AF647$485 / 100μlE11-7067A-AF680$485 / 100μlE11-7067A-AF700$485 / 100μlE11-7067A-AF750$485 / 100μlE11-7067A-AF790$485 / 100μlE11-7067A-APC$485 / 100μlE11-7067A-PE$485 / 100μlE11-7067A-Cy3$485 / 100μlE11-7067A-Cy5$485 / 100μlE11-7067A-Cy5.5$485 / 100μlE11-7067A-Cy7$485 / 100μlE11-7067A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.