All Names
AlternameEIF2S1, Eukaryotic translation initiation factor 2 subunit 1
All SitesHuman: Ser51; Mouse: Ser52; Rat: Ser51
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human eIF2α around the phosphorylation site of serine 51 (E-L-SP-R-R).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityeIF2α (phospho-Ser51) antibody detects endogenous levels of eIF2α only when phosphorylated at serine 51.
ReferencesXavier Saelens, et,al. (2001) J. Biol. Chem; 276: 41620-41628.
Hiroyuki Kubota,et,al.(2003) J. Biol. Chem; 278: 20457-20460.
Shijian Chu, et,al. (2006) Am J Physiol Lung Cell Mol Physiol; 291: L983-L992.
Eileen Connolly, et,al.(2006) Mol. Cell. Biol; 26: 3955-3965.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells