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EGFR (Phospho-Tyr869) Antibody
Datasheet
Catalog Number:
E11-7065AAmount:
100μg/100μlSwiss-Prot No.:
P00533Altername:
ERBB1, Epidermal growth factor receptor precursor, Receptor protein-tyrosine kinase ErbB-1, kinase EGFRAll Sites:
Human: Tyr869; Mouse: Tyr871; Rat: Tyr870Form of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 869 (K-E-YP-H-A).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
EGFR (Phospho-Tyr869) antibody detects endogenous levels of EGFR only when phosphorylated at tyrosine 869.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 ELISA: 1:20000References:
Inoue A, et al. (2005) PLoS Med; 2(1): e13.
Sun H, et al. (2004) EMBO J; 23(1): 100-110.
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720.
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674.
O’Rourke DM, et al. (1997) Proc Natl Acad Sci USA; 94(7): 3250-3255.
Goldstein DJ, et al. (1994) J Virol; 68(7): 4432-4441.
- Western blot analysis of extracts from A431 cells untreated or treated with EGF (40μM, 10mins), using EGFR (Ab-869) antibody (Line1 and 2) and EGFR (phospho-Tyr869) antibody (Line 3 and 4).
Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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