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Primary Antibodies

EGFR (Phospho-Tyr869) Antibody

E11-7065ACite ID: EN0025440DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 ELISA: 1:20000
P00533
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameERBB1, Epidermal growth factor receptor precursor, Receptor protein-tyrosine kinase ErbB-1, kinase EGFR
All SitesHuman: Tyr869; Mouse: Tyr871; Rat: Tyr870

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 869 (K-E-YP-H-A).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityEGFR (Phospho-Tyr869) antibody detects endogenous levels of EGFR only when phosphorylated at tyrosine 869.
ReferencesInoue A, et al. (2005) PLoS Med; 2(1): e13.
Sun H, et al. (2004) EMBO J; 23(1): 100-110.
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720.
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674.
O’Rourke DM, et al. (1997) Proc Natl Acad Sci USA; 94(7): 3250-3255.
Goldstein DJ, et al. (1994) J Virol; 68(7): 4432-4441.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from A431 cells untreated or treated with EGF (40μM, 10mins), using EGFR (Ab-869) antibody (Line1 and 2) and EGFR (phospho-Tyr869) antibody (Line 3 and 4).
Western blot analysis of extracts from A431 cells untreated or treated with EGF (40μM, 10mins), using EGFR (Ab-869) antibody (Line1 and 2) and EGFR (phospho-Tyr869) antibody (Line 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E11-7065A-Conjugate. Price: $485 / Size: 100μl.

E11-7065A-FITC$485 / 100μlE11-7065A-Biotin$485 / 100μlE11-7065A-HRP$485 / 100μlE11-7065A-AP$485 / 100μlE11-7065A-AF350$485 / 100μlE11-7065A-AF405M$485 / 100μlE11-7065A-AF488$485 / 100μlE11-7065A-AF514$485 / 100μlE11-7065A-AF532$485 / 100μlE11-7065A-AF555$485 / 100μlE11-7065A-AF568$485 / 100μlE11-7065A-AF405S$485 / 100μlE11-7065A-AF405L$485 / 100μlE11-7065A-AF546$485 / 100μlE11-7065A-AF594$485 / 100μlE11-7065A-AF610$485 / 100μlE11-7065A-AF635$485 / 100μlE11-7065A-AF647$485 / 100μlE11-7065A-AF680$485 / 100μlE11-7065A-AF700$485 / 100μlE11-7065A-AF750$485 / 100μlE11-7065A-AF790$485 / 100μlE11-7065A-APC$485 / 100μlE11-7065A-PE$485 / 100μlE11-7065A-Cy3$485 / 100μlE11-7065A-Cy5$485 / 100μlE11-7065A-Cy5.5$485 / 100μlE11-7065A-Cy7$485 / 100μlE11-7065A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.