All Names
AlternameERBB1, Epidermal growth factor receptor precursor, Receptor protein-tyrosine kinase ErbB-1, kinase EGFR
All SitesHuman: Tyr869; Mouse: Tyr871; Rat: Tyr870
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 869 (K-E-YP-H-A).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityEGFR (Phospho-Tyr869) antibody detects endogenous levels of EGFR only when phosphorylated at tyrosine 869.
ReferencesInoue A, et al. (2005) PLoS Med; 2(1): e13.
Sun H, et al. (2004) EMBO J; 23(1): 100-110.
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720.
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674.
O’Rourke DM, et al. (1997) Proc Natl Acad Sci USA; 94(7): 3250-3255.
Goldstein DJ, et al. (1994) J Virol; 68(7): 4432-4441.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells