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Primary Antibodies

EGFR (Phospho-Tyr1197) Antibody

E11-7064ACite ID: EN0025438DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:10000
P00533
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameERBB1, Epidermal growth factor receptor precursor, Receptor protein-tyrosine kinase ErbB-1, kinase EGFR
All SitesHuman: Tyr1197; Mouse: Tyr1197; Rat: Tyr1196

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 1197 (A-E-YP-L-R).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityEGFR (phospho-Tyr1197) antibody detects endogenous levels of EGFR only when phosphorylated at tyrosine 1197.
ReferencesCorbalan-Garcia S, et al. (1996) Mol Cell Biolt; 16(10): 5674-5682.
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720.
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674.
O’Rourke DM, et al. (1997) Proc Natl Acad Sci USA; 94(7): 3250-3255.
Goldstein DJ, et al. (1994) J Virol; 68(7): 4432-4441.
Price225$
Immunohistochemical analysis of paraffin-embedded Western human breast carcinoma tissue using EGFR (Phospho- or Tyr1197) antibody. (Ab-1197) Tyr1197)
Immunohistochemical analysis of paraffin-embedded Western human breast carcinoma tissue using EGFR (Phospho- or Tyr1197) antibody. (Ab-1197) Tyr1197)
blot analysis of extracts from A431 cells untreated treated with EGF (40μM, 10mins), using EGFR antibody (Line 1 and 2) and EGFR (phospho- antibody (Line 3 and 4).
blot analysis of extracts from A431 cells untreated treated with EGF (40μM, 10mins), using EGFR antibody (Line 1 and 2) and EGFR (phospho- antibody (Line 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E11-7064A-Conjugate. Price: $485 / Size: 100μl.

E11-7064A-FITC$485 / 100μlE11-7064A-Biotin$485 / 100μlE11-7064A-HRP$485 / 100μlE11-7064A-AP$485 / 100μlE11-7064A-AF350$485 / 100μlE11-7064A-AF405M$485 / 100μlE11-7064A-AF488$485 / 100μlE11-7064A-AF514$485 / 100μlE11-7064A-AF532$485 / 100μlE11-7064A-AF555$485 / 100μlE11-7064A-AF568$485 / 100μlE11-7064A-AF405S$485 / 100μlE11-7064A-AF405L$485 / 100μlE11-7064A-AF546$485 / 100μlE11-7064A-AF594$485 / 100μlE11-7064A-AF610$485 / 100μlE11-7064A-AF635$485 / 100μlE11-7064A-AF647$485 / 100μlE11-7064A-AF680$485 / 100μlE11-7064A-AF700$485 / 100μlE11-7064A-AF750$485 / 100μlE11-7064A-AF790$485 / 100μlE11-7064A-APC$485 / 100μlE11-7064A-PE$485 / 100μlE11-7064A-Cy3$485 / 100μlE11-7064A-Cy5$485 / 100μlE11-7064A-Cy5.5$485 / 100μlE11-7064A-Cy7$485 / 100μlE11-7064A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.